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KMID : 0613820110210101466
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Journal of Life Science
2011 Volume.21 No. 10 p.1466 ~ p.1472
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Characterization of the Promoter Controling the Stage-Specific Gene Expression of Bombyx mori
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Park Seung-Won
Choi Gwang-Ho
Goo Tae-Won
Kim Seong-Ryul
Kang Seok-Woo
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Abstract
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We characterized embryo early gene (EEG)-704 promoter of the silkworm Bombyx mori, which is specifically regulated in the development stages. To determine core promoter region, 10 different partial mutant clones were tested by luciferase assay in Sf9 cells. About 1.5 kb promoter shows higher luciferase activity than constitutive promoter (BmA3). Interestingly, EEG-704 shares the same DNA sequences with BmHsp20.8 by the result of BLAST analysis; its expression is also increased under heat shock condition. Development of such promoter inducible, directly or indirectly in the developmental-stage, is very useful in making recombinant proteins in transgenic silkworms.
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KEYWORD
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Bombyx mori, embryo early gene, promoter, luciferase assay
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